Research Square

Factor H related 2 levels dictate FHR dimer composition

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6130401/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the established paradigm of complement biology, specifically focusing on the role of Factor H and Factor H-Related proteins in regulating the alternative pathway of complement activation. Highlights This preprint focuses on refining the understanding of the dynamic interactions and dimerization of FHR proteins, particularly […]

Factor H related 2 levels dictate FHR dimer composition Read More »

Transcriptional landscape of canine hematopoiesis and cross-species comparisons revealed by single-cell RNA sequencing

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6299609/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of molecular biology and genomics, specifically focusing on single-cell RNA sequencing as a tool to understand cellular differentiation and lineage commitment. It also adheres to the established paradigm of using model organisms (dogs) to study human diseases. Highlights This research utilizes

Transcriptional landscape of canine hematopoiesis and cross-species comparisons revealed by single-cell RNA sequencing Read More »

Donor-reactive T cells and innate immune cells promote pig-to-human decedent xenograft rejection

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6474835/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of transplantation immunology, specifically xenotransplantation. This paradigm acknowledges the organ shortage crisis and explores the use of animal organs as a potential solution. The current paradigm emphasizes the significant challenge posed by the immune response, both adaptive and innate, which leads

Donor-reactive T cells and innate immune cells promote pig-to-human decedent xenograft rejection Read More »

MDA5 guards against infection by surveying cellular RNA homeostasis

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6466919/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of immunology, specifically the framework of innate immune sensing of viral infections via pattern recognition receptors (PRRs). Highlights This research refines the current understanding of MDA5 activation. The prevailing model suggests that MDA5, a PRR, is activated by long viral dsRNA.

MDA5 guards against infection by surveying cellular RNA homeostasis Read More »

AI-Driven CRISPR-Cas9 sgRNA Design for PDCD1, TRAC, and B2M Knockout to Improve CAR T Cell Therapy

Leave a Comment / Normal Science, Research Square /

URL https://assets-eu.researchsquare.com/files/rs-6596407/v1/83cfb5f0-6d5a-4363-be23-415570bc1a7c.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of gene editing using CRISPR-Cas9 technology for CAR T-cell therapy. It adheres to the established methodology of sgRNA design and validation, focusing on improving existing techniques rather than proposing radical shifts. Highlights This preprint demonstrates normal science by refining established techniques

AI-Driven CRISPR-Cas9 sgRNA Design for PDCD1, TRAC, and B2M Knockout to Improve CAR T Cell Therapy Read More »

The Role of Blood-Brain Barrier Dysfunction in Mediating Autoantibody and Immune Cell Entry in Pediatric Anti-NMDA Receptor Encephalitis: A Comparative Study

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6649929/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of neuroimmunology, specifically focusing on the role of blood-brain barrier dysfunction in pediatric anti-NMDA receptor encephalitis. This paradigm accepts the well-established understanding of the immune system's role in neurological disorders and the importance of the blood-brain barrier in maintaining CNS homeostasis.

The Role of Blood-Brain Barrier Dysfunction in Mediating Autoantibody and Immune Cell Entry in Pediatric Anti-NMDA Receptor Encephalitis: A Comparative Study Read More »

Celiac disease in Afghanistan: prevalence of an existing but unknown disease to Afghans

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6675314/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of biomedical research, specifically within the established framework of immunology and gastroenterology as they pertain to Celiac Disease. It adheres to standard diagnostic procedures and utilizes established laboratory techniques. Highlights This preprint represents a typical example of normal science. The research

Celiac disease in Afghanistan: prevalence of an existing but unknown disease to Afghans Read More »

The Influenza-like Evolutionary Path of Respiratory RNA Viruses

Leave a Comment / Model Drift, Research Square /

URL https://www.researchsquare.com/article/rs-6707035/v1.pdf Stage Model Drift Paradigm framing The preprint operates predominantly within the established epidemiological paradigm of infectious disease modeling, exemplified by the SIR (Susceptible-Infected-Recovered) model. However, it introduces modifications that challenge certain assumptions of this paradigm, suggesting a state of Model Drift. Highlights This preprint introduces the SIRS-G model, an extension of the SIRS

The Influenza-like Evolutionary Path of Respiratory RNA Viruses Read More »

Current Therapeutic Strategies for Rasmussen Encephalitis: A Systematic Review of Functional Hemispherectomy and Immunotherapy in Pediatric Intractable Epilepsy

Leave a Comment / Model Drift, Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6689486/v1.pdf Stage Normal Science / Model Drift Paradigm framing The dominant paradigm is the immunological understanding of Rasmussen Encephalitis (RE), encompassing the role of cytotoxic T-cells, inflammation, and the rationale for immunomodulatory therapies. The preprint also acknowledges the surgical paradigm, where hemispherectomy is the most effective intervention for seizure control. Highlights This systematic review

Current Therapeutic Strategies for Rasmussen Encephalitis: A Systematic Review of Functional Hemispherectomy and Immunotherapy in Pediatric Intractable Epilepsy Read More »

3D imaging of human pancreas suggests islet size and endocrine composition influence their loss in type 1 diabetes

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6759572/v1.pdf Stage Normal Science Paradigm framing The current paradigm in Type 1 diabetes (T1D) research centers around the autoimmune destruction of insulin-producing β-cells in the pancreas. This preprint works within this established paradigm. Highlights This research utilizes advanced 3D imaging techniques (light sheet fluorescent microscopy) to investigate the size, composition, and distribution of pancreatic

3D imaging of human pancreas suggests islet size and endocrine composition influence their loss in type 1 diabetes Read More »