Transcriptional landscape of canine hematopoiesis and cross-species comparisons revealed by single-cell RNA sequencing

The preprint operates within the dominant paradigm of molecular biology and genomics, specifically focusing on single-cell RNA sequencing as a tool to understand cellular differentiation and lineage commitment. It also adheres to the established paradigm of using model organisms (dogs) to study human diseases.

This research utilizes established methodologies (scRNA-seq, flow cytometry) and analytical approaches to investigate canine hematopoiesis. The study focuses on characterizing cell types, gene expression dynamics, and lineage relationships within a well-defined system. The comparative analysis with human data strengthens the existing paradigm of cross-species comparisons in hematopoiesis research. While the study expands our knowledge of canine hematopoiesis, it does not challenge or seek to overturn existing paradigms. It contributes to the accumulation of knowledge within the current framework of normal science, making incremental progress rather than revolutionary breakthroughs. There is a possibility that the discovered divergent gene expression patterns between canine and human granulopoiesis could be a starting point for future research exploring potential paradigm shifts in specific areas of hematopoietic regulation or disease modeling, but currently, the work is firmly grounded in normal science.