Research Square

Donor-reactive T cells and innate immune cells promote pig-to-human decedent xenograft rejection

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6474835/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of transplantation immunology, specifically xenotransplantation. This paradigm acknowledges the organ shortage crisis and explores the use of animal organs as a potential solution. The current paradigm emphasizes the significant challenge posed by the immune response, both adaptive and innate, which leads […]

Donor-reactive T cells and innate immune cells promote pig-to-human decedent xenograft rejection Read More »

MDA5 guards against infection by surveying cellular RNA homeostasis

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6466919/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of immunology, specifically the framework of innate immune sensing of viral infections via pattern recognition receptors (PRRs). Highlights This research refines the current understanding of MDA5 activation. The prevailing model suggests that MDA5, a PRR, is activated by long viral dsRNA.

MDA5 guards against infection by surveying cellular RNA homeostasis Read More »

AI-Driven CRISPR-Cas9 sgRNA Design for PDCD1, TRAC, and B2M Knockout to Improve CAR T Cell Therapy

Leave a Comment / Normal Science, Research Square /

URL https://assets-eu.researchsquare.com/files/rs-6596407/v1/83cfb5f0-6d5a-4363-be23-415570bc1a7c.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of gene editing using CRISPR-Cas9 technology for CAR T-cell therapy. It adheres to the established methodology of sgRNA design and validation, focusing on improving existing techniques rather than proposing radical shifts. Highlights This preprint demonstrates normal science by refining established techniques

AI-Driven CRISPR-Cas9 sgRNA Design for PDCD1, TRAC, and B2M Knockout to Improve CAR T Cell Therapy Read More »

The Role of Blood-Brain Barrier Dysfunction in Mediating Autoantibody and Immune Cell Entry in Pediatric Anti-NMDA Receptor Encephalitis: A Comparative Study

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6649929/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of neuroimmunology, specifically focusing on the role of blood-brain barrier dysfunction in pediatric anti-NMDA receptor encephalitis. This paradigm accepts the well-established understanding of the immune system's role in neurological disorders and the importance of the blood-brain barrier in maintaining CNS homeostasis.

The Role of Blood-Brain Barrier Dysfunction in Mediating Autoantibody and Immune Cell Entry in Pediatric Anti-NMDA Receptor Encephalitis: A Comparative Study Read More »

Celiac disease in Afghanistan: prevalence of an existing but unknown disease to Afghans

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6675314/v1.pdf Stage Normal Science Paradigm framing The preprint operates within the dominant paradigm of biomedical research, specifically within the established framework of immunology and gastroenterology as they pertain to Celiac Disease. It adheres to standard diagnostic procedures and utilizes established laboratory techniques. Highlights This preprint represents a typical example of normal science. The research

Celiac disease in Afghanistan: prevalence of an existing but unknown disease to Afghans Read More »

The Influenza-like Evolutionary Path of Respiratory RNA Viruses

Leave a Comment / Model Drift, Research Square /

URL https://www.researchsquare.com/article/rs-6707035/v1.pdf Stage Model Drift Paradigm framing The preprint operates predominantly within the established epidemiological paradigm of infectious disease modeling, exemplified by the SIR (Susceptible-Infected-Recovered) model. However, it introduces modifications that challenge certain assumptions of this paradigm, suggesting a state of Model Drift. Highlights This preprint introduces the SIRS-G model, an extension of the SIRS

The Influenza-like Evolutionary Path of Respiratory RNA Viruses Read More »

Current Therapeutic Strategies for Rasmussen Encephalitis: A Systematic Review of Functional Hemispherectomy and Immunotherapy in Pediatric Intractable Epilepsy

Leave a Comment / Model Drift, Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6689486/v1.pdf Stage Normal Science / Model Drift Paradigm framing The dominant paradigm is the immunological understanding of Rasmussen Encephalitis (RE), encompassing the role of cytotoxic T-cells, inflammation, and the rationale for immunomodulatory therapies. The preprint also acknowledges the surgical paradigm, where hemispherectomy is the most effective intervention for seizure control. Highlights This systematic review

Current Therapeutic Strategies for Rasmussen Encephalitis: A Systematic Review of Functional Hemispherectomy and Immunotherapy in Pediatric Intractable Epilepsy Read More »

3D imaging of human pancreas suggests islet size and endocrine composition influence their loss in type 1 diabetes

Leave a Comment / Normal Science, Research Square /

URL https://www.researchsquare.com/article/rs-6759572/v1.pdf Stage Normal Science Paradigm framing The current paradigm in Type 1 diabetes (T1D) research centers around the autoimmune destruction of insulin-producing β-cells in the pancreas. This preprint works within this established paradigm. Highlights This research utilizes advanced 3D imaging techniques (light sheet fluorescent microscopy) to investigate the size, composition, and distribution of pancreatic

3D imaging of human pancreas suggests islet size and endocrine composition influence their loss in type 1 diabetes Read More »

Trajectory of thirst level in critically ill oncology patients: A group-based trajectory model

Leave a Comment / Normal Science, Research Square /

URL https://assets-eu.researchsquare.com/files/rs-6488220/v1_covered_bf14d4c6-0551-46a0-bc69-380fce1a703d.pdf Stage Normal Science Paradigm framing The paradigm framing is thirst assessment and management in critically ill patients, particularly those in oncology. The dominant paradigm accepts the importance of thirst management and investigates its prevalence, intensity, and related factors in the ICU setting. This study operates within this paradigm by investigating thirst level trajectories

Trajectory of thirst level in critically ill oncology patients: A group-based trajectory model Read More »

Exploring the use of Virtual Reality to enhance rapid decompression from occupational stress during short wellness breaks for content moderators

Leave a Comment / Normal Science, Research Square /

URL https://assets-eu.researchsquare.com/files/rs-5684444/v1_covered_6871fded-8bc3-44c1-a101-5f10e11abdc8.pdf Stage Normal Science Paradigm framing Psychology, Occupational Health, Human-Computer Interaction Highlights This preprint investigates the application of Virtual Reality (VR) for stress reduction in a specific occupational group (content moderators). While the study did not demonstrate significant changes in long-term, trait-based anxiety or occupational stress, it did show reductions in short-term, state-based stress

Exploring the use of Virtual Reality to enhance rapid decompression from occupational stress during short wellness breaks for content moderators Read More »