Donor-reactive T cells and innate immune cells promote pig-to-human decedent xenograft rejection

The preprint operates within the dominant paradigm of transplantation immunology, specifically xenotransplantation. This paradigm acknowledges the organ shortage crisis and explores the use of animal organs as a potential solution. The current paradigm emphasizes the significant challenge posed by the immune response, both adaptive and innate, which leads to rejection. The research in this preprint investigates these immunological barriers, focusing on the role of T cells, NK cells, and other immune components.

This preprint represents normal science within the xenotransplantation paradigm. It utilizes established experimental methods like mixed lymphocyte reactions, flow cytometry, and high-throughput sequencing to investigate specific aspects of the immune response in a pig-to-human decedent xenotransplant model. The study focuses on characterizing the dynamics of donor-reactive T cells and the contribution of innate immune cells to rejection. While the findings provide valuable insights and contribute to the existing body of knowledge within the field, they do not challenge the fundamental assumptions of the paradigm. The research aims to refine our understanding of the immune mechanisms involved in xenograft rejection, with the ultimate goal of improving immunosuppression strategies and enhancing the success of xenotransplantation. No anomalies or fundamental crises within the paradigm are highlighted.